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Nutritional CRO & Prüflabor

BioTeSys
Publikationen.

BioTeSys publiziert regelmäßig wissenschaftliche Artikel zu unterschiedlichen Themen aus dem Umfeld der Prüfung und wissenschaftlichen Absicherung von biologischen und chemischen Produkten.

Publikation: 07. März 2015

Health Claims for Food Supplements and Degree of Evidence

While assessing the applications for possible health claims for food constituents, we advocate the integration of additional elements, namely grading of evidence, consideration of the risk-benefit ratio and epidemiological and health economic factors.

Publikation: 09. September 2014

Mathematical modelling of the automated FADU assay for the quantification of DNA strand breaks and their repair in human peripheral mononuclear blood cells.

DNA strand breaks are one of the most common genotoxic lesions and they can also arise as intermediates of DNA repair activity. We have previously published an automated method for measuring DNA strand breaks based on fluorimetric detection of alkaline DNA unwinding [1], and here we present a mathematical model of the FADU assay, which enables to an analytic expression for the relation between measured fluorescence and the number of strand breaks.

Publikation: 27. Mai 2014

Perilla extract improves gastrointestinal discomfort in a randomized placebo controlled double blind human pilot study

Gastrointestinal (GI) discomfort, e.g. bloating or rumbling, is a common symptom in otherwise healthy adults. Approximately 20% of the population, particularly women suffer from gastrointestinal discomfort and this affects quality of life. Recent studies discovered a link between the body and mind, called the gut-brain axis. Psychosocial factors, such as e.g. daily stress may cause altered gut physiology leading to ileum contractions and consequently gastrointestinal symptoms. In vitro and ex vivo studies clearly showed that a Perilla frutescens extract combines prokinetic, antispasmodic and anti-inflammatory effects. The aim of the intervention was to investigate the effects of the proprietary Perilla extract on GI discomfort in healthy subjects with gastrointestinal discomfort and reduced bowel movements in comparison to a placebo product.

Publikation: 14. Dezember 2013

The use of total antioxidant capacity as surrogate marker for food quality and its effect on health is to be discouraged

Attempts have been made to use non-compositional parameters, such as total antioxidant capacity (TAC), determined by assays such as oxygen radical absorbance capacity, ferric-reducing ability of plasma, and trolox-equivalent antioxidant capacity, as surrogate markers for food quality and for monitoring food-related changes in human plasma in dietary intervention studies. Increased TAC of plasma is often indiscriminately, and therefore incorrectly, interpreted as being favorable to human health. Whether or not dietary compounds may indeed exert health effects depends on factors other than mere presence in food or body fluids. Many phytochemicals, for example, are poorly absorbed and rapidly metabolized into molecules with altered physicochemical, and therefore biological, properties. Consequently, the use of TAC assays for the in vitro assessment of antioxidant quality of food, which often is employed as a marketing argument or for the assessment of the "wholesomeness" of food, is to be discouraged.

Publikation: 29. Mai 2013

High-resolution quantitative metabolome analysis of urine by automated flow injection NMR.

Metabolism is essential to understand human health. To characterize human metabolism, a high-resolution read-out of the metabolic status under various physiological conditions, either in health or disease, is needed. Metabolomics offers an unprecedented approach for generating system-specific biochemical definitions of a human phenotype through the capture of a variety of metabolites in a single measurement. The emergence of large cohorts in clinical studies increases the demand of technologies able to analyze a large number of measurements, in an automated fashion, in the most robust way. NMR is an established metabolomics tool for obtaining metabolic phenotypes.

Publikation: 26. März 2013

Physiological effects of oral glucosamine on joint health: current status and consensus on future research priorities

The aim of this paper was to provide an overview of the current knowledge and understanding of the potential beneficial physiological effects of glucosamine (GlcN) on joint health. The objective was to reach a consensus on four critical questions and to provide recommendations for future research priorities. To this end, nine scientists from Europe and the United States were selected according to their expertise in this particular field and were invited to participate in the Hohenheim conference held in August 2011. Each expert was asked to address a question that had previously been posed by the chairman of the conference. Based on a systematic review of the literature and the collection of recent data, the experts documented the effects of GlcN on cartilage ageing, metabolic/kinetic and maintenance of joint health as well as reduction of risk of OA development. After extensive debate and discussion the expert panel addressed each question and a general consensus statement was developed, agreeing on the current state-of-the-art and future areas for basic and clinical studies. This paper summarizes the available evidence for beneficial effects of GlcN on joint health and proposes new insight into the design of future clinical trials aimed at identifying beneficial physiological effect of GlcN on joint tissues.

Publikation: 01. September 2012

Investigation of a Perilla frutescens special extract – Anti-inflammatory and immune-modulatory properties

Perilla frutescens (L.) Britton leaves are used as food and in traditional medicine. A Perilla leaf special extract was used to investigate the anti-inflammatory effects and mode of immune-modulatory properties in the following ex vivo experiments addressing different immune cells. After stimulation, pattern of cytokines were determined and thus information about mode of action derived. As a result, anti-inflammatory effects were seen on the monocytic level with significant reduction of TNF-s production whereas on the T-helper cellular level, reactions were less pronounced but a shift towards a TH2-helper response was identified. Both effects were dose dependent. Results underline the anti-inflammatory and other immune-modulatory properties which might impact the beneficial effects in different indications, like for example irritable bowel syndrome.

Publikation: 01. Juni 2012

Essenzielle Mikronährstoffe zur Verbesserung des Versorgungsstatus bei gesunden Probanden

Der Nutzen einer Mikronährstoffsupplementation wird in der Wissenschaft kontrovers diskutiert. Dennoch gibt es Hinweise darauf, dass verschiedene Erkrankungen durch geringe Mikronährstoffdefizite begünstigt werden. In einer randomisierten, placebokontrollierten, doppelblinden Studie wurde der Einfluss einer Nahrungsergänzung mit einer breiten Kombination von aufeinander abgestimmten Mikronährstoffen bei 48 gesunden Probanden über 3 Monate untersucht. Ziel war es, Informationen sowohl zur extra- als auch intrazellulären Versorgungslage zu erhalten. Die Ergebnisse zeigen, dass eine solche Supplementation den Versorgungsstatus auch in gesunden Personen unterstützen und dabei einzelne Stoffwechselparameter positiv beeinflussen kann.

Publikation: 04. November 2011

The Synergistic Antioxidate Effect of Ascorbyl 2-Phosphate and Alpha-Tocopheryl Acetate

The use of antioxidants in cosmetics has a long history. Two different modes of application can be distinguished. They are used either to stability the cosmetic formulation or to protect the skin. As a formulation stabilizer, they serve as a "technical antioxidant." Here they are being used to prevent the oxidative deterioration of labile ingredietns such as unsaturated fatty acids or oxidation-prone active ingredeitns. Both synthetic compounds like butylated hydroxytoluene and natural antioxidants like alpha-tocopherol are frequently used for this purpose. The synergistic antioxidative effect of ascorbyl 2-phosphate and alpha-tocopheryl acetate was investigated in vitro using a human keratinocyte cell line and a fluorescence-based assay system.

Publikation: 19. Oktober 2011

The automated FADU-assay, a potential high-throughput in vitro method for early screening of DNA breakage.

Genotoxicity tests are essential to identify compounds that have a potential to compromise not only the environment but also human and animal health, including compounds that increase the risk of cancer. At present, no single test is capable of detecting all types of genotoxic effects; therefore a battery of in vitro and, if positive, in vivo tests is necessary to determine the genotoxicity of a substance. However, the respective specificities of current assays have been criticized for their high percentage of false positive results. We recently published an automated version of the "Fluorimetric detection of Alkaline DNA Unwinding" (FADU) assay for measuring DNA strand breaks in human peripheral blood mononuclear cells or in cell lines.